Anti-KLH, anti-SRBC, anti-tetanus toxoid, anti-DNP and anti-TNP IgM and IgG are routinely used as biomarkers of immunotoxicity. Life Diagnostics manufactures ELISA kits for these important biomarkers in mice, rats, chickens, and monkeys. In preclinical studies, animals are immunized with an immunogen such as KLH while being dosed with a drug candidate. Serum is collected at either 7 or ~ 21 days and the levels of anti-KLH IgM and IgG are determined and compared with those in a control group that was not exposed to the drug. A decrease in anti-KLH levels in the treatment group provides evidence of immunosuppression.

Orders may be placed by phone, fax, email, or via our online shop.

For technical advice please email us at techsupport@lifediagnostics.com. All kits listed below are manufactured by Life Diagnostics, Inc. All kits lare manufactured by Life Diagnostics, Inc.

Our rat and monkey anti-KLH IgG ELISA kits have recently been evaluated and gained unqualified approval in a respected independent laboratory (Validation of an ELISA assay for quantification of anti-KLH IgG in rat and monkey sera. CIT, Evreux, France. Armelle N. Guyen, Sandrine Arthaud, Phillipe Ancian and Roy Forster. Toxicology Letters, Volume 180, Supplement 1, 5 October 2008, Page S211).

Publications describing the use of LDI Immunotoxicity kits

1. Guyen AN, Arthaud S, Ancian P and Foster R. Validation of an ELISA assay for quantification of anti-KLH IgG in rat and monkey sera. Toxicology Letters (Abstracts) 180S: S211/I11 (2008)
2. Betz BC, Jordan-Williams KL, Wang C, Kang SG, Liao J, Logan MR, Kim CH and Taparowsky EJ. Batf coordinates multiple aspects of B and T cell function required for normal antibody responses. J. Exp. Med. 207(5):933-942 (2011) [Mouse anti-SRBC IgG & IgM] http://jem.rupress.org/content/207/5/933.full.pdf+html
   
3. Schaffer BS, Grayson MH and Wortham JM, et al. Immune competency of a hairless mouse strain for improved preclinical studies in genetically engineered mice. Mol. Cancer Ther. 9:2354-2364 (2010) [Mouse anti-KLH IgG & IgM] http://mct.aacrjournals.org/content/9/8/2354.full.pdf+html
   
4. Efanov A, Zanesi N, NazaryanN, Santanam U, Palmarchuck A Croce CM and Pekarsky Y. CD5+CD23+ leukemic cell populations in TCL1 transgenic mice show significantly increased proliferation and Akt phosphorylation. Leukemia 24:970-975 (2010) [Mouse anti-SRBC IgM]
5. Nain S and Smits JEG. Pathological, immunological and biochemical markers of subchronic arsenic toxicity in rats. Environmental Toxicology. (2010) http://onlinelibrary.wiley.com/doi/10.1002/tox.20635/abstract  [Rat anti-KLH IgG & IgM]
6. Zhou X, Xiang Y, Ding, X and Garrard WT. Anew hypersensitive site, HS10, and the enhancers, E3′ and Ed, differentially regulate Igk gene expression. J. Immunol. 188:2722-2732 (2012) [Mouse anti-KLH IgG & IgM]
7. Sah S and Smits J. Dietary selenium fortification: a potential solution to chronic arsenic toxicity. Toxicological & Environmental Chemistry. 94(7): 1453-1465 (2012) [Rat anti-KLH IgG]
8. Anderson SE, Franko J, Anderson KL, Munson AE, Lukomska E, Meade BJ. Immunotoxicity and allergic potential induced by topical application of dimethyl carbonate (DMC) in a murine model. J Immunotoxicol. [Epub ahead of print] (2012) [Mouse anti-SRBC IgM]
9. Aulí M, et al. Multiparametric immunotoxicity screening in mice during early drug development. Toxicol Lett. [Epub ahead of print] (2012) [Mouse anti-KLH IgM]
   
10. Sah S and Smits J. Treating chronic arsenic toxicity with high selenium lentil diets. Toxicol Appl Pharmacol. doi: 10.1016/j.taap.2013.06.008. (2013) [Rat anti-KLH IgG]